dkrc Chapter 379 The Last Step!
Chapter 379 The Last Step!
People kept coming to see the boss with microscopes in hand.
It was okay at first, but after watching it dozens of times in a row, Song He suddenly felt strange. He seemed to be a testing instrument. Whenever everyone made a set of samples, they would queue up and stuff them into the instrument for testing.
Fortunately, this strange feeling did not last long, and his attention was quickly attracted to the cell samples!
quick! The progress is too fast!
The cell status seen under the microscope is completely different every time. Even without looking at the data pop-up from the system, just observing with the naked eye, you can see that the cell status is changing rapidly!
In the afternoon, breakthroughs began to appear one after another!
From time to time, cheers erupted in the laboratory to celebrate the completion of the differentiation of a type of corneal cells.
Continuous battles and consecutive victories!
After dinner, Song He called everyone to the whiteboard and held a short meeting.
"Share the progress and experience of each group today!" Song He said concisely, "Lou Jinghui?"
"Our group's matrix layer is 80% complete!" Lou Jinghui reported proudly.
"The adhesive substances and keratinocytes composed of keratin sulfate and cartilage sulfate have been made separately!"
"The current problem is how to make collagen fibers into sheets, and how to combine these materials tightly and make them uniform and translucent!"
"The new experience our group has learned is the infiltration method!" Lou Jinghui turned to look at Ji Tianhe, "I saw Ji Tian and their group, after preparing the corneal differentiation solution, poured it on roughly, like watering flowers. But this will cause cells The mortality rate is increasing, and we use a slow infiltration method!"
Lou Jinghui went on to talk about a few operating points, and everyone applauded. Song He simply threw a series of small red flowers over.
"Ji Tianhe!" Song He called his name.
"We have a large group, so we are supporting each group today!" Ji Tianhe reported seriously, "The main achievement so far is to make wing-shaped cells of the epithelial cell layer!"
"The new experience is the photothermal method, which uses light beams to heat the culture medium, which can avoid the disturbance of traditional heating and improve the survival rate of cells!"
The crowd applauded, and Song He threw a series of small red flowers over to reward Ji Tian and the group.
"Professor Lu?" Song He looked at the white-haired old man in the crowd.
"The endothelial layer is successfully completed!" Lu Chengen said happily.
"The main experience of our group is pH! Gradual changes in pH can effectively promote cell differentiation. However, we haven't figured out the specific rules yet, so we can only oscillate the pH in small amplitudes!"
Song He continued to reward little red flowers, and this time he emptied all the pockets of his clothes.
"The progress on my side is also very smooth!" Song He said, "I am responsible for the epithelial cell layer, including basal cells and flat cells. As for the wing cells, Ji Tianhe's team made them, and the three types of cells were gathered together. Got it!"
"The only difficulty at the moment is the formation of the plasma membrane by the bridge body, but we have some clues, and I think we can complete the breakthrough tonight!"
"I think the progress of each group is almost there. I will work overtime tonight and try to finish the fifth floor in one go!"
"You guys are so fast! It's far beyond my expectations. Maybe we can take an early vacation!"
Song He waved his hands with satisfaction and everyone dispersed to work.
The atmosphere in the laboratory was warm and filled with joy on the eve of victory!
Sure enough, just after twelve o'clock in the evening, each group finished one after another, and all five cornea layers were completed!
A black technology blueprint appeared in front of Song He's eyes, and the progress bar was pushed forward to 80%!
There is one last step left!
[Splicing: Configure splicing fluid to closely combine the five corneal layers, modify the thickness and shape, and ensure that the approximation to the real cornea reaches more than 90%]
After reading the blueprint requirements, Song He immediately recalled the question book in his mind!
Five types of corneal layers were successfully made, and the question book can answer dozens of questions!
After answering, there were only a few questions left in the question book with answers to them, which happened to be the formula for the splicing fluid of the five-layer cornea!
The ingredients of the formula undoubtedly come from the few remaining little notes! Not only are there ingredient names, they even have percentage ranges listed!
The answer is ready!
Song He was in an excited mood. The question book and the black technology blueprint given by the Wishing Well matched each other. It was like a double cheat. The effect of the cheat became stronger as it went further!
He works hands-on, carefully configuring the splicing solution, just like a perfume master completing his career breakthrough!
Use a machine to stack five fresh corneas together, pour in the splicing fluid, and watch!
There are five thin transparent corneal layers, but their composition is extremely complex, including basal cells, wing cells, flat cells, desmoid plasma membrane, collagen fibers, keratin sulfate, cartilage sulfate, keratinocytes, hexagonal endothelial cells...
It’s hard to imagine how, over hundreds of millions of years, the cornea of the eye evolved step by step into the structure it has today.
When they are stacked together, they look like layers of burgers, but the thickness is measured in microns.
Fortunately, with the splicing fluid, it's like adding a big dollop of salad dressing and ketchup to the burger, making it sticky!
Each layer gradually merges, and the cells are closely connected to form a seamless whole!
Song He's heartbeat quickened as he operated the instrument to slowly polish the artificial cornea. He tried hard to restrain his hand shaking. He was as nervous as a jeweler polishing a rare diamond. A slight mistake would destroy the treasure!
Seeing the boss's actions, everyone in the laboratory also guessed that the end was near, and hurriedly gathered around, not daring to leave the ground to watch quietly, waiting to witness a miracle!
After only a dozen seconds of gentle polishing, the black technology blueprint popped up in front of my eyes!