dkrc Chapter 709 How To Make Red Blood Cells
Chapter 709 How To Make Red Blood Cells
As soon as Song He entered the laboratory door early in the morning, his face was distorted with fear.
More than half of the team members have already gone to work, and there are also more than a dozen students, including the provincial champion. Everyone is neatly dressed in white coats, gathering together to roll up their sleeves... and draw blood!
More than a dozen bags of blood have been drawn out, and more people are applying iodophor on their arms. The nurse in charge of drawing blood is Fan Tao. She is not good at her skills. The test table is covered with mist-like blood stains, and the scene is bloody. Mosaic is required.
"What are you doing?" Song He was confused.
"Get experimental materials." Fan Tao said, "Each person donates 100 ml and extracts some stem cells."
"Is our laboratory so poor that we can't afford mice? Where's my money?" Song He was stunned, "Why do we need to use human blood when we have mice? With this approach, will I have to recruit a large number of new people soon?"
"The air transport flight for the mice was delayed and had to be delivered at night. Everyone felt that we couldn't do nothing all day. Let's try it with some human blood first. Anyway, sooner or later we will use human blood." Fan Tao said calmly, "Boss, do you want to contribute? a little?"
"Ridiculous! No more smoking! Do you have a fucking medical qualification certificate?" Song He decisively stopped, "The health workers must have guinea pigs, I'll call them to borrow them."
Song He was sweating a little. While calling Xiang Wenwei, the dean of the Department of Health and Engineering, he reflected on whether there was something wrong with the way he led the team. The team members were acting more and more crazy, and even used their own blood as experimental materials. horrifying scene.
Xiang Wenwei was very talkative and immediately expressed his good-neighborly friendship. All the mice stored by Sanggong could be delivered free of charge. Fan Tao drove someone to pick up the goods and pulled out more than a dozen boxes of mice. Many teachers and students in the Sanggong laboratory had expressions on their faces. Displeased, but unable to disobey the dean's order.
Song He posted a chart on the wall of the laboratory, with large black characters that stood out:
[Step One: Red Blood Cells]
[Step 2: White blood cells]
[Step Three: Platelets]
[Step 4: Plasma microcomponents]
The four major steps are arranged in order of difficulty. The further you go, the more difficult it becomes. However, the simplest mass production of red blood cells is actually extremely difficult.
The information provided by the system is heart-wrenching, and a lot of key parts are missing. I only know two ways to go.
The first way is the stem cell method, which Song He handed over to Lu Chengen's team.
First, the blood of mice is extracted. About 500,000 stem cells can be isolated from every 500 ml. They are placed in a porous polyester-based simulated bone marrow structure. After difficult cultivation, they become 60 billion red blood cells. However, the yield rate is extremely low. After screening, There are less than 10 billion available.
The experiment was successful, but from a practical perspective it was a failure. Song He calculated that the final cost of producing red blood cells based on the stem cell method, even if scaled up, would be far higher than the price of human blood in various countries, at least 30 times. The price gap between left and right.
It's too expensive, and it's impossible to bring it to market at this price.
Lu Chengen's team continued to explore and try to find ways to reduce the cost. Song He no longer gave them instructions and allowed them to play freely. It didn't matter whether they could produce results or not.
The second way is the assembly method. Make hemoglobin and red blood cell outer membrane separately, and try to put them together like building blocks. Song He personally rolled up his sleeves for this route.
Hemoglobin is easy to make, just transfer the relevant genes into Escherichia coli. Escherichia coli is simply a gift from God to the biological laboratory. It produces hemoglobin obediently, and the yield is extremely high.
When making hemoglobin E. coli, Song He said that he could just do it by himself, but many team members insisted on working together. They felt that more people could make it faster.
The final result was a slap in the face. Song He easily produced a large amount of perfect hemoglobin in just two days. The team members who worked with him took four or five days and finally gave up in a hurry.
Everyone can’t understand why the boss can grow bacteria several times faster than others with the same equipment and reagents. It doesn't look like he has any unique skills. Is he just relying on illusory intuition?
The Holy Body of Innate Bacteria?
The production of red blood cell membranes is much more difficult. The red blood cell membranes of different blood types contain completely different polysaccharides and proteins, and there cannot be any deviation.
Professor Zhu from South China University of Technology and Academician Jeffrey from New Mexico State University jointly tried to make red blood cells. They used ready-made red blood cells as molds, coated them with silicon dioxide, covered them with positively and negatively charged polymers, and then etched away the silicon dioxide.
It works, but this kind of carving technique is also extremely expensive. Song He asked the students to try it. The price of the final product was at least more than 200 times the market price of human blood, making it completely unusable for commercial use. .
Another route that the academic community is exploring is liposomes of artificial phospholipids. It happens that this method is also included in the information provided by the system, and Song He personally started to fight.
The progress was unexpectedly smooth. After leading the team to try hundreds of substances, Song He figured out the trick. With the addition of dequinonium chloride and polyethylene glycol, special proteins and polysaccharides can be effectively inserted into artificial phospholipids to create shapes. Red blood cells of different blood types.
And after some estimation, the price is reasonable. After mass production, the cost price will be lower than that of human blood.
At this point, the assembly method works!
In the middle of the night, team members worked together to produce the first artificial mouse red blood cell sample on the assembly line. The total amount was one kilogram, and bright red flowed in the sample jar.
Everyone excitedly watched the bright red liquid in the sample jar, their faces glowing red, like a group of vampires getting a hard-earned dessert.
"Awesome, you can make it! You can make the mouse version, and you can make the human version too!"
"It should be the world's first mature artificial red blood cells, right?"
"Strictly speaking, yes, some seniors have tried to make it, but it was just a shapeless thing and it didn't work at all. Ours should work, and the oxygen content is perfect!"
"Quick! Find a guinea pig and try it!"
In full public view, six white mice were captured and injected with artificial red blood cells!
Then, die!
The death rate was very fast. Everyone thought it would take a long time for the results to come out. They were still chatting happily when they suddenly discovered that the white mice were dying one after another.
The smiles disappeared from everyone's faces and their faces became sad, but there was no time for silence. The only way to find out the true cause of death was to hurry up and do autopsies.
After working for most of the night, the cause of death was not found, the red blood cells were not adulterated with any toxic substances, the injection amount was appropriate, and the mouse had no internal injuries, but it just died.
"Do I have to pay my respects?" Some people were confused and couldn't help but wonder.
In line with the principle that no money is required for worshiping, everyone prayed devoutly, and then caught six white mice for injection experiments.
That changed this time.